Fig. 6. Knockdown POTEE could decrease tumor growth in vivo.

a Representative photographs showing the tumors in mice after the subcutaneous transplantation of RKO cells with POTEE knockdown or control cells. b Tumor growth curves measured at the indicated time points between POTEE knockdown and control groups. Statistics were measured by 2-way ANOVA, ^*P < .05, ^**P < .01, ^***P < .001. c Representative IHC photographs showed positive relationship between POTEE, SPHK1, p-p65, Ki67 expression in tumor tissues (left). Results of Ki67 immunohistochemistry staining were evaluated by the staining scores (right). Results are shown as mean ± SD (n = 6). ^*P < .05 based on Paired t-test. d. Schematic depiction of the mechanisms underlying CRC proliferation via POTEE/SPHK1/p65 signaling