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. 2019 Nov 7;10:2495. doi: 10.3389/fmicb.2019.02495

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Copyright © 2019 Ge, Long, Yuan, Pan, Shen and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphological observation shows that the ClpP-dependent level of CsrA affects entry into the RP. (A) Bacterial morphology of L. pneumophila observed with 100× magnification under oil at the indicated time points. The indicated strains in the TP were inoculated into AYE media to the same initial OD₆₀₀ of 0.2 with shaking at 37°C. (B–E) Cell length of WT/pJB908 (B), WT/pcsrA (C), ΔclpP/pJB908 (D), and ΔclpP/pcsrA (E) was calculated with ImageJ at the indicated time points. Data represent mean ± SEM derived from three independent experiments. **p < 0.01 were identified by GraphPad Prism. ns means no difference from the wild type.