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. 2019 Nov 7;10:2513. doi: 10.3389/fmicb.2019.02513

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Copyright © 2019 Orner, Bhattacharya, Kalenja, Hayden, Del Poeta and Fries.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Decreased lifespan of mutants and increased cell size with age. Replicative lifespans were determined for each mutant strain and compared to its respective wildtype (A,B). Cell sizes were measured in the young (0–3 generations) and old (10 generations) fractions of cells after separation to assess the cell size of each population (C,D). ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001 by one-way ANOVA. ^$p < 0.05, ^$$p < 0.01, ^$$$$p < 0.0001 by one-way ANOVA compared to wildtype counterpart.