TABLE 3.

Young cells and mutant strains are less virulent in Galleria mellonella.

Strain name	Age	Galleria survival (Median) days	P-value	Significance	Remarks
H99	Y	6	0.0015	S	Aging significantly increased virulence
	O	5
lac1Δ	Y	6	0.55	NS	Age-associated increased virulence disappeared
	O	6
app1Δ	Y	6	0.55	NS	App1 important in age-dependent resilience
	O	6
H99	Y	6	0.0943	NS	Lac2 compensates for Lac1 in young cells
lac1Δ	Y	6
H99	Y	6	0.5238	NS	App1 works via complement and Galleria lacks full complement system
app1Δ	Y	6
H99	O	5	0.0002	S	Lac2 failed to compensate for loss of Lac1 in old age
lac1Δ	O	6
H99	O	5	0.0022	S	App1 needed for age-dependent virulence
app1Δ	O	6
KN99α	Y	6	0.004	NS	Aging significantly increased virulence
	O	5
lac2Δ	Y	7	0.47	NS	Lac2 important in age-dependent resilience
	O	7
KN99α	Y	6	0.02	NS	Lac1 compensates for Lac2 in young cells
lac2Δ	Y	7
KN99α	O	5	0.0004	S	Lac1 failed to compensate for loss of lac2 in old age
lac2Δ	O	7