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. 2019 Nov 7;10:2562. doi: 10.3389/fmicb.2019.02562

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Copyright © 2019 Sinha-Ray, Alam, Bag, Morris and Ali.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PacBio whole genome sequencing and sequence comparison of Vibrio cholerae AAS91 to that of its mother strain Env-9 (NCBI: NZ_CP012997.1 and NZ_CP012998.1) corroborated the successful integration of the 21,153-bp of RCT prophages between rtxA and VC1479 in AAS91 following chitin-induced transformation. We compared only the large chromosome where RCT is present. While Env-9 lacked RCT prophages (inner chromosome), AAS91 acquired entire RCT prophages as presented in extended form with arrows showing the genes comprising of RCT prophages.