TABLE 1.
Bacterial strains and plasmids used in this study.
| Strain/Plasmid | Description/genotype | References |
| V. cholerae | ||
| E7946 | O1 El Tor, ogawa clinical toxigenic isolate in 1978 from Bahrain | Laboratory collection |
| N16961 | O1 El Tor, inaba clinical toxigenic isolate in 1971 from Bangladesh | Heidelberg et al., 2000 |
| O395 | O1 classical, ogawa clinical toxigenic isolate in 1965 from India | Laboratory collection |
| HC16 | O1 El Tor Ogawa clinical toxigenic isolate in 2012 from Haiti | Rahman et al., 2014 |
| HC1037 | O1 El Tor Ogawa clinical toxigenic isolate in 2014 from Haiti | Duncan et al., 2018 |
| Env-2 | Non-O1/Non-O139 environmental non-toxigenic isolate in 2012 from Haiti | Rahman et al., 2014 |
| Env-9 | O1 Ogawa environmental non-toxigenic isolate in 2012 from Haiti | Azarian et al., 2016 |
| AAS35 | Rifampicin resistant version of Env-9 created by spontaneous mutation, rifR | This study |
| AAS56 | E7946 Δ orfU::kanR | This study |
| AAS69 | AAS35 Δ xerC::kanR, rifR | This study |
| AAS70 | AAS35 Δ xerD::kanR, rifR | This study |
| AAS72 | E7946 Δ orfU::specR | This study |
| AAS74b | AAS35 Δ recA::kanR, rifR | This study |
| AAS91 | AAS35::RS1-CTX-TLCΔ orfU::kanR, rifR | This study |
| AAS93 | AAS35::RS1-CTX-TLCΔ orfU::specR, rifR | This study |
| AAS111 | AAS35::RS1-CTX-TLCΔ xerC::kanR, rifR | This study |
| AAS125 | E7946 Δ xerC::kanR | This study |
| E. coli | ||
| DH5α | recA Δ lacU169 ϕ80d lacZΔ M15 | Gibco, BRL |
| Plasmids | ||
| pBAD/His A | pBR322 origin, expression vector, ampR | Thermo fisher scientific |
| pSMA4 | 1065 bp recA ORF of E7946 was cloned into XhoI-EcoRI sites of pBAD/His A, ampR | This study |