FIG 5.

Shape and size measurements of R. parkeri bacteria under pressure from different drugs. (A) Representative bacterial cells from the EC curve drug concentrations from panel B. Scale bar, 1 μm. (B) Dose-dependent changes in area and eccentricity of R. parkeri bacterial cells under drug treatment with lovastatin, pitavastatin, tetracycline, or d-cycloserine. Each measurement is the mean of at least 40 individual bacterial cells, except for concentrations of d-cycloserine at >250 μM, where most bacterial cells were lysed and fewer measurements were possible (for 500 μM d-cycloserine, n = 22; for 1,000 μM d-cycloserine, n = 18; for 2,000 μM d-cycloserine, n = 13). Error bars represent the 95% confidence interval. (C) Graphs plotting area and eccentricity measurements at the EC₁₀₀ values calculated from the data shown in Fig. 4B and matched to measurements from panel A. Regions shaded in yellow are the calculated values of EC₅₀ to EC₁₀₀ from the data shown in Fig. 4B. Graphs show the mean of the sample set, and error bars represent the 95% confidence interval. Statistical comparisons were done by one-way ANOVA of results for all drug-treated samples compared to results for the no-drug (nd) sample set (ns, not significant; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). dcs, d-cycloserine; tet, tetracycline; lov, lovastatin; pit, pitavastatin.