Figure 1.

Experimental setup. (A) Inlet and outlet (access) channels as well as a central channel were drilled into the top glass layer. Channel networks were etched into the bottom layer, producing channels 190 μm in width and 70 μm in depth. The tissue chamber was sealed with a microport and PDMS-filled adaptor. The tissue chamber has a volume of approximately 20 μL. Adapted from Hattersley et al [34]. (B) Pictures of the chip. Note: Two outlet holes glued with glass coverslip. (C) A picture of the assembled microfluidic chip. (D) Schematic of microfluidic setup (Adapted from Dawson et al.) [60]. (E) The device with GBM tissue, connected to syringes and syringe pump.