Figure 3.
Impact of AaTI on DENV Infection
(A) AaTI mRNA level after RNAi depletion and oral infection. Mosquitoes were injected with dsRNA against AaTI (dsAaTI) or LacZ (dsLacZ) 4 days before oral infection. AaTI mRNA was quantified in midguts at the time of quantification of midgut infection and normalized to actin mRNA level. Each point represents one sample containing 10 midguts.
(B and C) (B) Infection rate in the midguts and (C) foci of infection per infected midgut of dsAaTI- and dsLacZ-injected mosquitoes 3 days post oral infection.
(D and E) (D) Infection rate in mosquitoes and (E) pfu per infected dsAaTI- or dsLacZ-injected mosquito 7 days post feeding on blood with or without rAaTI.
(A, C, and E) Each point represents one sample. Differences were tested with t test. (A and C) Lines show means ± SEM. (B and D) Bars show percentage ± SE. Differences were tested with χ2 test. n, number of mosquito samples. (E) Differences were tested with t test after log-transformation. Lines show geometric means ± 95% CI.