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. 2019 Nov 13;7(21):e14269. doi: 10.14814/phy2.14269

Figure 4.

Figure 4

Representative fluorescence images showing 4‐HNE staining (a marker of lipid peroxidation; Fig. 4A), and 8‐OHdG staining (a marker of oxidative DNA damage; Fig. 4C) in the LV sections. DAPI was used to stain nuclei. White arrow in Figure 4C showed 8‐OHdG stained cell (green) in the TAC LVs. Scale = 100 μm. (B and D) The fluorescence intensity of 4‐HNE (red), and number of 8‐OHdG stained cells (green) in the LV sections were quantitated by NIH‐ImageJ software and plotted as the mean ± SD. I.D. = Integrated density. Bar diagrams showing malondialdehyde (MDA) concentration (E) and glutathione (GSH) level (F) in the LV of sham, sham‐α‐CGRP, TAC, and TAC‐α‐CGRP mice after α‐CGRP delivery (28 days). Values were expressed as the mean ± SD and *P < 0.05 was considered significant. ns = non‐significant. (G) Western blots showing SOD‐2 and β‐actin protein level in total cell proteins extracted from sham, sham‐α‐CGRP, TAC, and TAC‐α‐CGRP LV tissues. The ratio SOD‐2/β‐actin was plotted as the mean ± SD. ns = non‐significant.