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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Bone Miner Res. 2019 Jul 31;34(11):2087–2100. doi: 10.1002/jbmr.3819

Figure 4: RNAseq analysis reveals two main transcriptional programs: one loading-responsive and the other driven by the NT3 transgene.

Figure 4:

(A) Schematic representing the tibial region of interest harvested for RNA-Seq analysis (top line: 2 mm distal to the articular surface and bottom line: distal tibia-fibula junction). (B) Heatmap displaying z-scores across all samples highlighting two main transcriptional programs: one induced by loading (blocks 1–2) and the other by the NT3 transgene (blocks 3–4). (C) Table listing RNAseq analysis comparisons and description of resulting differentially expressed genes (DEGs). (D-G) Volcano plots for each of the comparisons in (C) showing number of upregulated DEGs in each pairwise comparison. DEGs were defined as having a fold-change > 1.5 together with an adjusted p-value ≤ 0.05. n=4 per genotype/loading condition. light purple = Ctrl NL; dark purple = Ctrl LOAD; light green = NT3-Cre+ NL; dark green = NT3-Cre+ LOAD