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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Hypertension. 2019 Oct 21;74(6):1409–1419. doi: 10.1161/HYPERTENSIONAHA.119.13676

Figure 1. Prolonged exposure to high ET-1 did not impair EDR or reduce eNOS activity.

Figure 1.

A) Naïve aortic rings from female C57BL/6NHSD mice were cultured in VascuLife® cell culture media containing endothelin-1 (ET-1; 0nM-10nM) ± BQ788 (1μM, ETB receptor antagonist) for 48 hours. Aortic rings were mounted on steel pins in organ bath chambers and exposed to increasing concentrations of endothelin-1 (ET-1), acetylcholine (ACh), and sodium nitroprusside (SNP); n=8/condition. U-46619 (20nM) was used to preconstrict the arteries prior to the assessment of relaxation. B) Human aortic endothelial cells were treated with ET-1 (0nM–10nM) for 48 hours under standard cell culture conditions. eNOS activation (p-eNOS Ser1177/total eNOS) was determined via Western blot; n=5–6/condition. All data are expressed as means ± SEM. *p<0.05 vs Con or 0nM ET-1. Con, control; AUC, area under the curve.