Indel frequency and reads distribution of CRISPR/Cas9 off-target sites containing microsatellites in the control group. (A) Distribution of captured predicted off-target sites with different sequence features in the control group. (B) Distribution of the editing efficiency and reads in predicted off-target sites with different sequence features in the control group. (C) Distribution of the microsatellites in predicted off-target sites in 20, 19, 18, and 17 nt sgRNAs. NRG: protospacer adjacent motif (PAM); N = A, T, C, or G; R = A or G; STR: short tandem repeat; OT: predicted off-target site.