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. 2019 Oct 3;15(12):2641–2653. doi: 10.7150/ijbs.37152

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Validation of selected predicted off-target sites containing microsatellites. (A) Sequence and microsatellite features of potential off-target sites. (B) Detection of cleavage activity of the predicted off-target sites by T7ENI cleavage assay. Control represents negative control group. POT: potential off-target site; ID: identity number; NRG: protospacer adjacent motif (PAM); N = A, T, C, or G; R = A or G. Nucleotides marked in red and blue colors represent PAM and mismatches, respectively.