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. 2019 Oct 21;15(12):2750–2762. doi: 10.7150/ijbs.36916

Figure 4.

Figure 4

MTERFD1 is associated with irradiation sensitivity in CRC cells. (A, B) Effect of irradiation on MTERFD1 expression. (A) HT29 and SW1116 cells were irradiated with 0, 2, 4, or 8 Gy. The relative levels of MTERFD1 mRNA were determined via qRT-PCR using cells treated with 0 Gy as controls. (B) HT29 and SW1116 cells were irradiated with 4 Gy, and relative levels of MTERFD1 mRNA were determined via qRT-PCR at 0, 4, 8, 24 and 48 hours post-irradiation. (C, D) Effect of MTERFD1 expression on irradiation sensitivity. (C) HT29 and LOVO cells transfected with either MTERFD1-OE plasmid or control vector were irradiated with 4 Gy. Apoptosis was analyzed via flow cytometry 24 hours post-irradiation. (D) SW1116 cells transfected with MTERFD1 siRNA1, siRNA2 and control siRNA were irradiated with 4 Gy. Apoptosis of cells both with and without irradiation was analyzed via flow cytometry 24 hours post-irradiation. All experiments were performed in triplicate and a representative result is shown. Data are shown as the means ± S.D. *: p<0.05, **: p<0.01, ***: p<0.001.