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. 2019 Oct 15;15(12):2676–2691. doi: 10.7150/ijbs.35630

Figure 2.

Figure 2

MPAPO promotes axonal regeneration of the corneal nerve after injury in C57BL/6 mice. (A) MPAPO promotes axonal regeneration of the corneal nerve as shown by immunofluorescence staining. DAPI is the blue in the nucleus, and AF594 is an anti-mouse red fluorescent secondary antibody used to stain trigeminal nerve fibers (the primary antibody used is the trigeminal tag antibody III-β-tublin). Merge is the result of the colocalization of the two kinds of fluorescence. (B) Cross-cut immunofluorescence staining of the eye tissue revealed that MPAPO can bind to optic nerve cells and other retinal cells. DAPI is a nuclear dye with blue fluorescence. FITC is connected to MPAPO with green fluorescence. Cy5 is an anti-rabbit red fluorescent secondary antibody, and the primary antibody is the commonly used neuro-tag antibody NF 200. Merge is the result of the three fluorescent colocalizations.