Figure 3.

Reduced glucose transporter, Slc2a1, expression is associated with Rbpj loss during in vivo artificial decidualization. Glucose transporter mRNA and protein levels were investigated by RT-qPCR and immunohistochemistry, respectively, during artificial decidualization. (A) Slc2a1 was significantly induced in the decidual horns of the control and Rbpj c-KO mice compared to control horns. Slc2a1 mRNA expression was reduced in the control horn of Rbpj c-KO mice compared to control mice (n = 3-5/group). (B) Slc2a1 protein localization was significantly reduced in the decidual horn of Rbpj c-KO mice, particularly in the secondary decidual zone (n = 4-5/group). Data represented as Mean ± standard error of the mean (SEM). Comparisons were made using Two-Way ANOVA for more than 2 groups or using a Student t-test for 2 groups. *P < .05, **P < .01; Scale Bar = 100 μm; US indicates unstimulated, non-decidualized uterine horn; S, stimulated, decidualized uterine horn; SDZ, secondary decidual zone; PDZ, primary decidual zone.