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. 2019 Nov 7;30(11):1361–1370. doi: 10.1089/hum.2019.132

Figure 4.

Figure 4.

FAF quantification of lipofuscin/A2E accumulation of ABCA4 hybrid dual vector-treated and untreated ABCA4−/− mouse retinas. AAV hybrid dual vectors packaged in AAV8(Y733F) were subretinally injected at 1 month of age. One eye was injected while the contralateral eye was left untreated. Autofluorescence was measured with a Heidelberg Engineering cSLO HRA+OCT at monthly intervals after injection. (A, B) quantification of FAF as a function of months after vector treatment. (C, D) examples of FAF images from which the data in (A, B) were derived. (A, C) FAF of ABCA4−/− animals treated with the 5′ vector only. (B, D) FAF in 5′ plus 3′ dual vector-treated animals. Error bars represent the mean ± SEM. ★p < 0.05. A2E, N-retinylidene-N-retinylethanolamine; cSLO, confocal scanning laser ophthalmoscope; FAF, fundus autofluorescence; HRA, Heidelberg retinal angiograph; OCT, optical coherence tomography; SEM, standard error of the mean.