Table 1.
Oligonucleotide primers used to construct the ATP binding cassette transporter A4 dual vectors platforms
| Overlap Vectors | ||
|---|---|---|
| P1 | GCGGCGGCCGCCACCATGGGCTTCGTGAGACAG | NotI |
| P2 | GCGACTAGTGCTGGCATATGCTCTGTGC | SpeI |
| P3 | GCGGCGGCCGCGCTTACTGTCTCCGGTGGC | NotI |
| P4 | GCGGAGCTCTCAGTCCTGGGCTTGTCG | SacI |
| Transsplicing Vectors | ||
|---|---|---|
| P5 | AGATTTTTGAGCCCTGTGGC | (FseI) |
| P6 | CTGCACTAGTGTCGACAGAAACGCAAGAGTCTTCTCTGTCTCGACAAGCCCAGTTTCTATTGGTCTCCTTAAACCTGTCTTGTAACCTTGATACTTACCTGATAGGTCCTGAGCCTC | SalI |
| P7 | GACTGGCGGCCGCTCGCGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGGCATGCAGAGAAAGCTG | NotI |
| P8 | TGTTGTCTTCAGTTTCTAGATG | XbaI |
| Hybrid Vectors | ||
|---|---|---|
| P9 | GCTTGGTCGACGAAACGGTCCAGGCTATGTG | SalI |
| P10 | GCTTGGTCGACCCCCGGGTGCGCGGCG | SalI |
| P11 | CGTTCGCGGCCGCCCCCGGGTGCGCGGCG | NotI |
| P12 | CGTTCGCGGCCGCGAAACGGTCCAGGCTATGTG | NotI |
The restriction sites introduced through the primers are listed and underlined in the sequence (The FseI site is only partially contained in primer P5).