REAGENT or RESOURCE	Source	Identifier
Antibodies
PE-Cy7 Mouse Anti-Human CD19	BD Biosciences	Cat#341113_clone SJ25-C1
Alexa Fluor 488 goat anti-Human IgM	Invitrogen	Cat#A21215; RRID:AB_2535800
Goat anti-Human IgA FITC	Invitrogen	Cat#A18788; RRID:AB_2535565
Anti-Human CD3 PC5	Beckman Coulter	Cat#A07749
Anti-Human CD38 APC	BD Pharmingen	Cat#555462_clone HIT2 (RUO); RRID:AB_398599
Anti-Human CD14 PC5	Beckman Coulter	Cat#A07765
Anti Human CD27 BV650	BioLegend	Cat#302827_clone O323; RRID:AB_11124941
AffiniPure F(ab’)2 Fragment Goat Anti-Human IgA+IgG+IgM (H+L)	Jackson Immunoresearch	Cat#109-006-064; RRID:AB_2337548
AP-conjugated anti-human IgG secondary antibody	Southern Biotech	Cat#2040-04; RRID: AB_2795643
10E8 recombinant mAb	IRB and Pasteur; Huang et al., 2012	PMID:23151583; RRID:AB_2491067
HK20 recombinant mAb	IRB; Sabin et al., 2010	PMID:21124990; RRID:AB_2491018
4E10 recombinant mAb	IRB and Pasteur; Stiegler et al., 2001	PMID:11788027; RRID:AB_2491029
LN01 Recombinant mAb and All Variants	this paper	N/A
Mouse Anti-HIV-1 Gag FITC (Clone KC57)	Beckman Coulter	Cat#6604665
mGO53	Dr. Hugo Mouquet, Institut Pasteur, Paris; Wardemann et al., 2003	PMID:12920303
3BNC117	Produced by Dr. Hugo Mouquet; Scheid et al., 2011	PMID: 21764753; RRID:AB_2491033
10–1074	Produced by Dr. Hugo Mouquet; Mouquet et al., 2012	PMID: 23115339; RRID:AB_2491062
PGT128	Produced by Dr. Hugo Mouquet; Walker et al., 2011	PMID:21849977; RRID:AB_2491047
PGT151	Produced by Dr. Hugo Mouquet; Falkowska et al., 2014	PMID: 24768347
Bacterial and Virus Strains
HIV-1 PV strain CE1176	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
HIV-1 PV strain BJOX2000	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
HIV-1 PV strain X1632	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
HIV-1 PV strain 25710	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
HIV-1 PV strain TRO.11	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
HIV-1 PV strain CH119	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
HIV-1 PV strain 246F3	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
HIV-1 PV strain CE0217	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
HIV-1 PV strain CNE55	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
SVA-MLV_control PV	Fraunhofer Institute for Biomedical Engineering IBMT	N/A
Epstein Barr Virus	IRB	N/A
pCH058.c/2960	NIH AIDS Reagent Program	Cat#11856
pCH077.t/2627	NIH AIDS Reagent Program	Cat#11742
pNL(AD8)	NIH AIDS Reagent Program	Cat#11346
pYU2	NIH AIDS Reagent Program	Cat#1350
Standard panel of reference Env pseudoviruses	Huang et al., 2016	Multiclade Reference Panel PMID:27315479
OverExpress C41(DE3) chemically competent E. coli cells	Sigma-Aldrich	Cat#CMC0017
Ni-NTA Agarose	Qiagen	Cat#30210
Biological Samples
HIV-1 Infected ART Naïve Patient LNMCs	Hospital “Sacco” in Milan, Italy	N/A
Human serum	Etablissement Français du Sang	N/A
Chemicals, Peptides, and Recombinant Proteins
28amino acids long peptide gp41 MPER	Pepscan	N/A
Human recombinant IL-6	BD Pharmingen	Cat#550071
Human recombinant IL-21	ImmunoTools	Cat#11340215
Human recombinant IL-2	IRB	N/A
MPER-TM1 Peptide	Smart Bioscience	www.smart-bioscience.com
Fos-Choline-12	anatrace	Cat#F308
n-Octyl-β-D-glucosid n-Octyl-β-D-Glucopyranoside	anatrace	Cat#O311
PEG3350	Sigma-Aldrich	Cat#88276
PEG8000	Sigma-Aldrich	Cat#89510
HEPES	Sigma-Aldrich	Cat#H4034
Ethylene glycol	Carl Roth	Cat#6881.1
06:0 PS	Avanti Polar Lipids	Cat#840030
Critical Commercial Assays
ImmunoWELL cardiolipin (IgG) test	GenBio	Cat#3090
Pierce Rapid Gold BCA Protein Assay kit	Thermo Fisher Scientific	Cat#A53225PR
NK cell isolation kit	Miltenyi Biotec	Cat#130-092-657
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit	Invitrogen	Cat#L34965
ANA HEp-2 Test System	Zeus Scientific	Cat# FA2400
EZ-LinkNHS-PEG4-Biotinylation Kit	Thermo Scientific	Cat#21455
Deposited Data
MPER-TM1-LN01 complex	this paper	6SNC
MPER-TM1-LN01-PS complex	this paper	6SND
MPER-TM2-LN01 complex	this paper	6SNE
Experimental Models: Cell Lines
TZM-bl cell line	NIH AIDS Reagent Program	Cat#8129
TZM-bl FcγRI cell line	NIH AIDS Reagent Program	Cat#11798
Expi293F cells	Thermo Fisher Scientific	Cat#A14527
Mesenchimal stromal cells (MSC)	IRB	N/A
CEM-NKR-CCR5	NIH AIDS Reagent Program	Cat# 4376
Raji	ATCC	CCL-86
293T/17	ATCC	Cat# CRL-11268
Experimental Models: Organisms/Strains
Mouse: homozygous human FcRn (huFcRn) transgenic mice_Tg276 strain	Jackson Laboratory	JAX: 004919
Oligonucleotides
CpG 2006	Microsynth	N/A
Recombinant DNA
Plasmid: IgG1 expression vector	Tiller et al., 2008	PMID:17996249
Plasmid: Igk expression vector	Tiller et al., 2008	PMID:17996249
pMX-YU2-ENVΔCT- IRES-GFP	Provided by Dr. Hugo Mouquet, Pasteur	N/A
Plasmid: MPER-TM2 expression vector	This study	N/A
Software and Algorithms
Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/
CLC Main Workbench 8	QIAGEN	https://www.qiagenbioinformatics.com/products/clc-main-workbench/
IMGT-the International Immunogenetics Information System	Lefranc et al., 1999	http://www.imgt.org
XDS	Kabsch, 2010	PMID:20124693
Phaser	McCoy et al., 2007	PMID:19461840
COOT	Emsley et al., 2010	PMID:20383002
REFMAC	Murshudov et al., 1997	PMID:15299926
Phenix	Adams et al., 2010	PMID:20124702
SBGrid	Morin et al., 2013	N/A
Pymol	Warren DeLano	www.pymol.org
VMD (Version 1.9.4)	Humphrey et al., 1996	https://www.ks.uiuc.edu/Research/vmd/
NAMD (Version 2.13)	Phillips et al., 2005	https://www.ks.uiuc.edu/Research/namd/
CHARMM-GUI	Jo et al., 2008	http://www.charmm-gui.org
WebLogo	Schneider and Stephens, 1990	https://weblogo.berkeley.edu/logo.cgi
wwPDB OneDep System		https://deposit-pdbe.wwpdb.org
wwPDB Validation Service		https://validate.wwpdb.org/
BLI Data Analysis software (v11.1.0.25)	Pall FortéBio	https://www.moleculardevices.com
SBGrid Consortium	(Morin et al., 2013)	https://sbgrid.org/
Python 3.7		https://python.org
Biacore T200 Control Software		https://www.gelifesciences.com/biacore