Table 3.

Analytical Validation Findings from cfDNA Mutant Pool Diluted at 1%, 0.5%, and 0.2% Frequencies

CRC23 panel	CRC23 panel: cfDNA from individual cell lines		Total
	Positive	Negative
Mutant cfDNA 1% dilution
Positive	117	0	117
Negative	18	175,935	175,953
Total	135	175,935	176,070
Mutant cfDNA 0.5% dilution
Positive	86	0	86
Negative	49	175,935	175,984
Total	135	175,935	176,070
Mutant cfDNA 0.2% dilution
Positive	52	0	52
Negative	83	175,935	176,018
Total	135	175,935	176,070

Note that for orthogonal verification, variants obtained by independently sequencing each cfDNA of the mutant pool were used.

cfDNA, cell-free DNA.