Table 3.
Novel miRNAs identified in Lupinus luteus.
| miRNA ID | Sequence (5’–3’) | Size (nt) | Precursor (RNA-seq ID) | LP (nt) | MFE (kcal/mol) | Target Description (degradome/psRNAtarget) |
|---|---|---|---|---|---|---|
| Ll-miRn1 | TTGCCAATTCCACCCATGCCTA | 22 | TRINITY_DN58100_c0_g3_i1 | 125 | −59.90 | SUPPRESSOR OF GENE SILENCING 3 |
| Ll-miRn2 | TCACTCCAACTTTGACCTTCT | 21 | TRINITY_DN50576_c0_g2_i1 | 215 | −84.70 | 65-kDa microtubule-associated protein 7 |
| Ll-miRn3 | TGAAGAGGGAGGGAGACTGATG | 22 | TRINITY_DN77107_c0_g1 | 185 | −86.50 | SRC2 homolog |
| Ll-miRn4 | GTAGCACCATCAAGATTCACA | 21 | TRINITY_DN43941_c0_g1_i1 | 151 | −60.30 | RCC1 and BTB domain-containing protein 2 |
| Ll-miRn5 | TGGAATAGTGAATGAGACATC | 21 | TRINITY_DN52736_c3_g2_i2 | 102 | −38.70 | Probable cinnamyl alcohol dehydrogenase 9 |
| Ll-miRn6 | TGCTATCCATCCTGAGTTTCA | 21 | TRINITY_DN54182_c6_g1_i1 | 133 | −47.90 | Probable amino acid permease 7 |
| Ll-miRn7 | AGAGGTGTATGGCACAAGAGA | 21 | TRINITY_DN53175_c1_g6_i1 | 85 | −36.60 | Probable protein phosphatase 2C |
| Ll-miRn8 | TGAAGTGTTTGGGGGAACTCC | 21 | TRINITY_DN44441_c0_g1_i1 | 102 | −37.40 | ATP sulfurylase 1 |
| Ll-miRn9 | TCGGACCAGGCTTTATTCCTT | 21 | TRINITY_DN50586_c0_g1_i3 | 167 | −65.60 | Homeobox-leucine zipper protein REVOLUTA |
| Ll-miRn10 | ATGTTGTGATGGGAATCAATG | 21 | TRINITY_DN67022_c0_g1_i1 | 84 | −43.50 | CBL-interacting serine/threonine-protein kinase 6 |
| Ll-miRn11 | TAAAGACCTCATTCTCTCATG | 21 | TRINITY_DN31556_c0_g1_i1 | 130 | −62.80 | Vacuolar protein sorting-associated protein 62 |
| Ll-miRn12 | AGGTCATCTTGCAGCTTCAAT | 21 | TRINITY_DN52990_c2_g1_i5 | 71 | −36.84 | DNA-directed RNA polymerase I subunit 1 |
| Ll-miRn15 | TTCGGCTTTCTACTTCTCATG | 21 | TRINITY_DN54101_c8_g2_i10 | 156 | −66.20 | Transcription termination factor MTERF8 |
| Ll-miRn16 | AGTTCTTTAGATGGGCTGGACGCC | 24 | TRINITY_DN52523_c6_g2_i1 | 83 | −36.50 | Amino acid transporter AVT6A |
| Ll-miRn17 | TGTCTCATTCTCTATCTCAAG | 21 | TRINITY_DN51068_c0_g1_i2 | 142 | −64.30 | IST1-like protein |
| Ll-miRn18 | AATAGGGCACATCTCTCATGG | 22 | TRINITY_DN46596_c0_g1_i1 | 112 | −49.00 | E3 ubiquitin-protein ligase HOS1 |
| Ll-miRn19 | TCCAAAGGGATCGCATTGATTT | 22 | TRINITY_DN53637_c4_g2_i4 | 110 | −48.10 | AUXIN SIGNALING F-BOX 3 |
| Ll-miRn21 | TGAGCATGAGGATAAGGACGG | 21 | TRINITY_DN50271_c0_g3_i1 | 246 | −144.90 | Tetratricopeptide repeat protein 1 |
| Ll-miRn22 | TATCATTCCATACATCGTCTCG | 21 | TRINITY_DN50592_c0_g4_i2 | 80 | −33.60 | Putative disease resistance RPP13-like protein 1 |
| Ll-miRn24 | ATTGTCACTGTATCATTCACCATT | 24 | TRINITY_DN52987_c0_g1_i1 | 104 | −32.30 | Zinc finger CCCH domain-containing protein 55 |
| Ll-miRn25 | TGGTACAAAAAGTGGGGCAAC | 21 | TRINITY_DN48871_c3_g1_i9 | 151 | −43.90 | Nuclear transcription factor Y subunit A-9 |
| Ll-miRn26 | TGTTGTTTTCTGGTAAAAATA | 21 | TRINITY_DN58488_c1_g2_i4 | 99 | −33.80 | Auxin-responsive protein IAA27 |
| Ll-miRn27 | ATTAGATCATGTGGCAGTTTCACC | 24 | TRINITY_DN51506_c3_g2_i5 | 77 | −36.60 | U-box domain-containing protein 33 |
| Ll-miRn28 | TACGGGTGTCCTCACCTCTGAT | 22 | TRINITY_DN70730_c0_g1_i1 | 98 | −36.90 | ISWI chromatin-remodeling complex ATPase |
| Ll-miRn29 | TGGGATAGAGAGTGAGATACC | 21 | TRINITY_DN51068_c0_g1_i2 | 125 | −67.80 | Ethylene-responsive transcription factor ERF017 |
| Ll-miRn30 | TTCGTTTGTGTGCAGACTCTGT | 22 | TRINITY_DN57730_c1_g9_i2 | 105 | −42.70 | Endoribonuclease Dicer homolog 2 |
| Ll-miRn31 | GCGTACCAGGAGCCATGCATG | 21 | TRINITY_DN58934_c0_g4_i1 | 149 | −60.20 | Calcium-transporting ATPase 4 |
| Ll-miRn32 | AAGGGTTGTTTACAGAGTTTA | 21 | TRINITY_DN51330_c0_g1_i1 | 128 | −55.40 | 26S proteasome regulatory subunit 7 |