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S229A and S229D mutations altered intracellular distribution of NS5A with regard to NS3. Confocal immunofluorescence micrographs of NS3 (red) costained with S235 phosphorylated NS5A (pS235, green) in the T7-Huh7 cells transfected with wild-type (WT), S229A, or S229D mutant NS3-NS5B mutant constructs. The nuclei were stained with DAPI (blue). Boxes indicate areas enlarged.
