FIG 3.

General influenza virus dynamics in mouse cohorts inoculated with H1N1p (A), H3N2hu (B), or H3N2ca (C). Control C57BL/6 mouse samples are denoted as squares while CMAH^−/− mouse samples are shown as triangles. Top, quantitation of influenza virus genome copies (per RT-qPCR of M segment) for each virus was measured for stock virus and pooled lung homogenate (normalized to inoculum volume) to measure genomic bottleneck size at each passage. H3N2hu-inoculated mice lacked measurable genome copies in their lungs at first passage. Bottom, mouse weights were recorded during the course of infection. Figures are from the first passage of series 1 as a representative example. All virus-specific weight-loss phenotypes persisted during the course of each experimental passage and between repeat passage series. Only mice inoculated with H1N1p showed weight loss during the course of infection, with no significant variation between experimental groups.