FIG 5.

Whole-genome deep sequencing metrics and quality control. (A) Gel migration of products of IAV whole-genome RT-PCR shows specific bands of 8 genome segments. Products were present only in infected lung homogenates (+) and absent in uninfected controls (−). RT-PCR mixtures generally contained >100,000 input genome copies. (B) Reads across genome segments were not equal, with frequent bias toward smaller segments. (C) Median reads across the genome segments show some variation in coverage across PB2/PB1/PA segments. Blue line, H1N1p; green line, H3N2ca.