FIG 4.

MA-HBR mutations alter VLP release efficiency. (A) HeLa cells were transfected with WT Gag-YFP or Gag-YFP containing 25/26KR, 25/26KT, 29/31KR, or 29/31KT changes. At 14 h posttransfection, cell and virus-like particle (VLP) lysates were collected and subjected to SDS-PAGE, and Gag proteins were detected by immunoblotting using HIV immunoglobulin. (B) Relative VLP release efficiency representing the amount of VLP-associated Gag as a fraction of the total Gag present in VLP and cell lysates and normalized to the VLP release efficiency of WT Gag. Results from 4 independent experiments are shown as means ± standard deviations. The average VLP release efficiency of WT Gag was 8.3%. P values were determined from raw data using Student’s t test. *, P ≤ 0.05; ****, P ≤ 0.0001.