FIG 8.

29/31KR GagLZ-YFP fails to release VLPs. (A) HeLa cells were transfected with Gag-YFP or GagLZ-YFP containing WT MA or 29/31KR MA sequences. At 16 h posttransfection, cell and VLP lysates were collected and analyzed as described in the legend of Fig. 4. (B) Relative VLP release efficiency representing the amount of VLP-associated Gag as a fraction of total Gag present in VLP and cell lysates and normalized to the VLP release efficiency of WT Gag. Results from 3 independent experiments are shown as means ± standard deviations. The average VLP release efficiency of WT Gag was 9.6%. ns, not significant; ***, P ≤ 0.001.