FIG 10.

Reovirus activates STAT1 and STAT2 signaling, and topoisomerase inhibitors activate DNA damage response pathways. (A) MDA-MB-231 cells were treated with the vehicle (DMSO) or 2 μM doxorubicin, epirubicin, or topotecan for 1 h; infected with reovirus at an MOI of 100 PFU/cell; and incubated with DMSO or 1 μM topoisomerase inhibitors for 0 to 2 days postinfection. Whole-cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies specific for phosphorylated and total STAT1, STAT2, STAT3, ATM, p53, and GAPDH and reovirus. Residues recognized by phosphorylation-specific antibodies are shown in parentheses. (B and C) Quantitative densitometry of immunoblots from three independent experiments. All data are normalized to values for GAPDH and DMSO (mock) for each corresponding day. Error bars indicate SEM.