FIG 11.

Topoisomerase inhibitors and r2Reovirus induce higher levels of IFNL1 over time than either agent alone. MDA-MB-231 cells were treated with the vehicle (DMSO) or 2 μM doxorubicin, epirubicin, or topotecan and infected with mock or r2Reovirus at an MOI of 100 PFU/cell. (A to C) RNA was isolated from cells at times shown, and qPCR was performed to assess mRNA levels of IFNB1 (A), IFNL1 (B), and reovirus S1 (C). The dashed line in panel C represents background baseline levels observed for mock. Data are shown as fold changes normalized to the value for a housekeeping gene for duplicate independent experiments. Error bars indicate SEM. (D) Levels of IFN-λ in cell supernatants, determined by an ELISA. Data are shown as picograms per milliliter of IFN-λ for duplicate independent experiments.