Genetic composition of r1Reovirus and r2Reovirus. The genetic composition of parental and reassortant r1Reovirus and r2Reovirus was determined by Illumina next-generation sequencing. r1Reovirus has seven gene segments from T1L and three from T3D (L2, M2, and S2) and four nonsynonymous point mutations (L3, A160T; S3, P161T I250V; S4, V49I). r2Reovirus has nine gene segments from T1L and one from T3D (M2) and three nonsynonymous point mutations (L3, A160T; S3, I250V; S4, V49I). Both r1Reovirus and r2Reovirus have several synonymous point mutations.