FIG 6.

Screening of NIH Clinical Collection small molecules for reovirus infectivity. MDA-MB-231 cells were treated with the vehicle (DMSO), 4 μM E64-d, or 10 μM compounds from the NIH Clinical Collection for 1 h and infected with r2Reovirus at an MOI of 20 PFU/cell in the presence of DMSO, 2 μM E64-d, or 5 μM compounds from the NIH Clinical Collection for 20 h. Cells were scored for infectivity by indirect immunofluorescence using reovirus-specific antisera. (A) Infectivity from average Z scores for compounds in the NIH Clinical Collection for duplicate experiments. (B) Compounds from the NIH Clinical Collection that increase (green) (top) or decrease (red) (bottom) infectivity by a Z-score of 2 or more. Data are shown as Z-scores for two independent experiments (run) and the average from both experiments.