TABLE 3.
Strains and phages used in this study
| Strain or phage (reference) | Description or propagating strain (reference or source) |
|---|---|
| Strains | |
| C. jejuni 12661 | Phage propagation strain (18) |
| C. jejuni 11168 MP21 | Phage propagation strain (22) |
| C. jejuni 12664 | Phage propagation strain (18) |
| E. coli WT MG1655(pBAD24) | Can synthesize queuosine-tRNA (17) |
| E. coli MG1655 ΔqueC(pBAD24) | Cannot synthesize queuosine-tRNA (17) |
| E. coli MG1655 ΔqueD(pBAD24) | Cannot synthesize queuosine-tRNA (27) |
| E. coli MG1655 ΔqueE(pBAD24) | Cannot synthesize queuosine-tRNA (17) |
| E. coli MG1655 ΔfolE(pBAD24) | Cannot synthesize queuosine-tRNA (17) |
| E. coli MG1655 ΔqueC(pBAD24)/queC | Complement expressing the queC homolog gp114 from phage CP220 (this study) |
| E. coli MG1655 ΔqueD(pBAD24)/queD | Complement expressing the queD homolog gp069 from phage CP220 (this study) |
| E. coli MG1655 ΔqueE(pBAD24)/queE | Complement expressing the queE homolog gp017 from phage CP220 (this study) |
| E. coli MG1655 ΔfolE(pBAD24)/folE | Complement expressing the folE homolog gp124 from phage CP220 (this study) |
| Phages | |
| NCTC 12673 (18) | C. jejuni 12661 (18) |
| NCTC 12669 (18) | C. jejuni 12664 (18) |
| F336 (15) | C. jejuni 11168 MP21 (22) |
| CP220 (28) | C. jejuni 12661 (18) |
| CPt10 (28) | C. jejuni 12661 (18) |