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Cryo-EM screening of PEDV CV777 S in the presence of a sialic acid analog. A representative cryo-EM micrograph of PEDV CV777 S in the presence of 2 mM 3′-sialyllactose taken at ×92,000 magnification is shown on the left. 2D class averages of the spike, calculated using cisTEM software (49), are shown at the top right. Side and top views of the 10-Å reconstruction, calculated in cisTEM by imposing C3 symmetry, are shown at the bottom right.
