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. 2019 Nov 13;93(23):e00986-19. doi: 10.1128/JVI.00986-19

FIG 8.

FIG 8

The Q4R mutation accelerates the kinetics of reverse transcription of the RGDA/Q112D virus. (A) Jurkat cells were treated with 0 or 200 U per ml of IFN-β for 16 h prior to infection. Cells were infected with VSV-G-pseudotyped HIV-1 isolates encoding the GFP reporter gene. The reverse transcriptase inhibitor nevirapine was added at the indicated time after infection. The percentage of GFP-positive cells was determined at 2 days after infection. Relative infectivity (compared with that for cells not treated with nevirapine [in percent]) was calculated by dividing the percentage of GFP-positive cells among nevirapine-treated cells by the percentage of GFP-positive cells among untreated cells. ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05. (B) The influence of IFN-β treatment on reverse transcription (RT) kinetics was analyzed by using the values presented in panel A. The mean values of the relative infectivity of cells treated with nevirapine at 4 h after infection from three independent experiments are shown with standard errors of the mean (SEM). *, P < 0.05.