Figure 3.

Subretinal injection of HDAd5-CMVp-eGFP induces cellular infiltration into the vitreous by 3 days postinjection. (A) Representative (n = 8; 4 males and 4 females, injected in two independent cohorts) panoramic confocal image depicting site of injection of HDAd5-CMVp-eGFP (eGFP; green) at 3 days postinjection. Transduction and eGFP expression are observed in the RPE and throughout the neural retina (A’, A’’). DAPI (blue) was used to counterstain cell nuclei. (B, C) Panoramic confocal images displaying an entire section through a buffer-injected (B, B’) and an HDAd5-injected (C, C’) eye at 3 days postinjection labeled with Iba1 (red) and the nuclear counterstain, DAPI (blue). White asterisks in (C) denote large clusters of vitreous cells, and white arrowheads (C’) point to the presence of Iba1-positive cells within the outer nuclear layer in a vector-treated eye. The solid white line in each inset demarcates the boundary between the GCL and the vitreous (Vit). Scale bars: (A) 400 μm, (A’, A’’) 100 μm, (B, C) 1000 μm, and (B’ C’) 100 μm. CH, choroid; Iba1, ionized calcium-binding adapter molecule 1; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; POS, photoreceptor outer segments; RPE, retinal pigmented epithelium.