Decreasing the dose of HDAd5-CMVp-eGFP by 3 log units abrogates inflammatory cellular infiltration, but also yields no visible retinal transduction. (A–C) Representative (n = 3 eyes injected per dose) confocal images at 3 days postinjection near the site of injection of HDAd5-CMVp-eGFP (eGFP; green) at each of three different doses: 5 × 109 vg (A), 5 × 108 vg (B), and 5 × 107 vg (C). Each image is labeled with anti-Iba1 (red), anti-ICAM-1 (gray), and the nuclear counterstain, DAPI (blue). Scale bars = 100 μm. Definition of abbreviations for retinal layers is the same as Fig. 3.