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. 2019 Nov 7;30(11):1405–1418. doi: 10.1089/hum.2019.019

FIG. 1.

FIG. 1.

miR-200c involved in mouse craniofacial development and osteogenic differentiation of hBMSCs. (A) μCT images of 7-week-old mice of transgenic PMIS-miR-200c and WT; bar = 1 mm. (B) Quantitative measurement of BV/TV using μCT at different locations of skull of transgenic mice with PMIS-miR-200c compared with WT mice. *p < 0.05, n = 3. (C) Fold change of miR-200c in hBMSCs after osteogenic differentiation. (D) Fold change of ALP, Runx2, and OCN in hBMSCs with endogenous miR-200c inhibited by PMIS 7 days after osteogenic differentiation. *p < 0.05 versus untreated, performed in triplicate. μCT, microcomputed tomography; ALP, alkaline phosphatase; BV/TV, bone volume/tissue volume; hBMSCs, human bone marrow mesenchymal stromal cells; miR, microRNA; OCN, osteocalcin; PMIS, plasmid-based microRNA inhibitor system; Runx2, Runt-related transcription factor 2; WT, wild type. Color images are available online.