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. 2019 Nov 7;30(11):1405–1418. doi: 10.1089/hum.2019.019

FIG. 3.

FIG. 3.

miR-200c promotes osteogenic differentiation of hBMSCs in vitro. (A) Transcripts of Runx2, ALP, and OCN of hBMSCs with different transfection 4 and 7 days after exposure to osteogenic support medium. (B, C) Photographs of ALP staining and the quantitative measurement of ALP activity in hBMSCs pretransfected with pDNA miR-200c. (D, E) Fold change of Runx-2 and its concentration in the supernatant of hBMSCs pretransfected with pDNA miR-200c after osteogenic differentiation. (F, G) Fold change of OCN and its concentration in the supernatant of the hBMSCs after osteogenic differentiation. (H, I) Photographs of ARS and quantitative CPC extraction of ARS staining hBMSCs pretransfected with miR-200c after osteogenic differentiation. *p < 0.05 versus untreated, performed in triplicate. ARS, Alizarin red staining; CPC, cetylpyridinium chloride; mRNA, messenger RNA; OD, optical density. Color images are available online.