Skip to main content
. 2019 Nov 7;30(11):1405–1418. doi: 10.1089/hum.2019.019

FIG. 6.

FIG. 6.

miR-200c targets Sox2 and Klf4 and upregulates Wnt activity. (A) The sequence and miR-200c binding region located in the 3′-UTR of Sox2 and Klf4. (B) Fold change of expression of miR-200c, 200a, and 15a in hBMSCs treated with pDNA miR-200c, miR-200a, or the same amount of scrambled miRs. (C) Fold change of Sox2 and Klf4 in hBMSCs treated with pDNA miR-200c, miR-200a, or the same amount of scrambled miRs. (D) Normalized luciferase activities of the 3′-UTR of Sox2 and Klf4-luciferase reporters treated with EV, miR-210, miR-200c, and PMIS-miR-200c. *p < 0.05 versus untreated; performed in triplicate. (E) TOP/FOPflash luciferase activity of HEPM cells with overexpression of miR-200c and scrambled miRs with or without Sox2. *p < 0.05 versus scrambled miR; #p < 0.05; performed in triplicate. (F) A Western blot analysis of Sox9 in untreated HEPM cells and the cells with miR-200c overexpression and scrambled miRs. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HEPM, human embryonic palatal mesenchyme; Klf4, Kruppel-like factor 4; Sox2, SRY (sex determining region Y)-box 2; UTR, untranslated region. Color images are available online.