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. 2019 Oct 30;20(6):4867–4874. doi: 10.3892/mmr.2019.10785

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Expression of ZEB2 in hyperoxia-exposed lung tissue. Neonatal rats were exposed to normoxic or hyperoxic conditions for 14 days; hyperoxia exposed rats were subsequently injected with shRNA-NC or shRNA-PLGF. (A) Western blot analysis and (B) quantification was performed to determine ZEB2 protein expression levels in rat lung tissues; β-actin was used as the endogenous control. Data are presented as the mean ± standard deviation. n=8. **P<0.01. (C) ZEB2 mRNA expression levels were detected by reverse transcription-quantitative PCR. Data are presented as the mean ± standard deviation. n=3. **P<0.01. NC, negative control; PLGF, placental growth factor; shRNA, short hairpin RNA; ZEB, zinc-finger E-box binding homeobox 2.