Figure 1.

H₂O₂ induces apoptosis and affects the expression of miR-455 in HESCs. (A) Flow cytometry analysis of the apoptosis rates in HESCs exposed to various doses of H₂O₂ for 24 h. (B) HESCs were treated with various concentrations of H₂O₂ for 24 h, and then, cellular lysates were collected and subjected to western blot analysis with the caspase-3 antibody. The histograms (right) show the densitometric analysis of the caspase-3 and cleaved caspase-3 western blot results. (C) HESCs were treated with various concentrations of H₂O₂, after which total cellular lysates were collected and subjected to a caspase-3/7 activity assay. (D) HESCs were treated with various concentrations of H₂O₂ for 24 h, and then, the levels of miR-455 were measured by qPCR. All data are shown as the mean ± SD of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. HESCs, human endometrial stromal cells; H₂O₂, hydrogen peroxide.