Figure 5.

Silencing of FABP4 protects HESCs from apoptosis induced by H₂O₂ in HESCs. (A) HESCs were transfected with si-NC or si-FABP4 for 24 h, and then, the mRNA levels of FABP4 were measured by qPCR. (B) HESCs were transfected with si-NC or si-FABP4 for 24 h. The protein levels of FABP4 were then assessed by western blot analysis. (C) HESCs were treated as indicated for 24 h, which was followed by a CCK-8 assay to detect cell viability. (D) The apoptosis rate of HESCs following the different treatments. (E) HESCs were treated as indicated, after which the total cellular lysates were subjected to western blot analysis with the caspase-3 antibody. The histogram shows the densitometric analysis of the caspase-3 (right panel) and cleaved caspase-3 (bottem panel) western blot results. (F) HESCs were treated as indicated, after which the relative caspase-3/7 activities were assayed. All data are shown as the mean ± SD of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. FABP4, fatty acid binding protein 4; H₂O₂, hydrogen peroxide; HESCs, human endometrial stromal cells.