Figure 3.

Curcumin suppresses apoptosis-associated protein expression in PQ-damaged WI-38VA13 cells. WI-38VA13 cells were treated with PQ (10 µmol/l) for 48 h, followed by a further 48-h incubation with curcumin (300 µmol/l). (A-D) Expression levels of Bcl-2 and Bax as determined by western blotting and reverse transcription-quantitative polymerase chain reaction. GAPDH was used as an internal control. Data are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 vs. control; ^{^}P<0.05, ^{^^}P<0.01 vs. PQ; ^#P<0.05, ^##P<0.01 vs. PQ + Cur. Cur, curcumin; PQ, paraquat.