Figure 5.

Curcumin modulates signaling pathways in PQ-treated WI-38VA13 cells. (A) Relative mRNA expression levels of Notch1 as determined by reverse transcription-quantitative polymerase chain reaction. (B) Protein expression levels of Notch1, p-ERK1/2 and ERK1/2 as detected via western blotting. (C) Protein expression levels of Notch1. (D) Ratio of p-ERK1/2 to ERK1/2. GAPDH was used as an internal control. Data are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 vs. control; ^{^^}P<0.01 vs. PQ; ^##P<0.01 vs. PQ + Cur. Cur, curcumin; ERK1/2, extracellular signal-regulated kinase 1/2; p, phosphorylated; PQ, paraquat.