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. 2019 Oct 9;20(6):5134–5144. doi: 10.3892/mmr.2019.10733

Figure 2.

Figure 2.

Ang II promotes apoptosis of podocytes. The apoptosis of podocytes was determined via flow cytometry after treatment with Ang II at concentrations of 10−9, 10−8, 10−7 and 10−6 mol/l. (A) Apoptotic rates of podocytes were significantly increased after treatment with Ang II from 10.43±0.74, 15.80±1.12, 19.69±1.59 and 25.17±0.91%, respectively, when compared with the control (9.25±0.81%). *P>0.05, #P<0.05 vs. Control. (B) Cell apoptosis of podocytes after treatment with Ang II at 10−6 mol/l for different time intervals (12, 24 and 48 h) as determined by flow cytometry. The apoptotic rates of podocytes were significantly increased after treatment with Ang II from 13.50±0.66% (12 h), 19.80±1.08% (24 h) and 27.16±1.39% (48 h) when compared with 0 h (8.37±0.71%). #P<0.05 vs. Control. The data are presented as the mean ± standard deviation from three independent experiments. For cell apoptosis analysis, statistical significance was determined one-way ANOVA followed by the Least Significant Difference test. Ang II, angiotensin II; FITC, fluorescein isothiocyanate; PI, propidium iodide.