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. 2019 Oct 9;20(6):5134–5144. doi: 10.3892/mmr.2019.10733

Figure 4.

Figure 4.

Ang II decreases the phosphorylation of Akt and inactivates p65 NF-κB in podocytes. Ang II reduced the expression of p-Akt and p-p65 NF-κB. Representative western blots of p-Akt and p-p65 NF-κB for 48 h expression in 10−6 M Ang II-treated podocytes are presented. Compared with the control group, Ang II decreased the phosphorylation levels of Akt and p65 NF-κB. #P<0.05 vs. Control. Compared with the Ang II incubated group, the combination of Ang II + 740Y-P restored the phosphorylation levels of Akt and NF-κB. *P<0.05 vs. Ang II. Stimulation of podocytes with Ang II + LY294002 further reduced the phosphorylation levels of Akt and p65 NF-κB vs. Ang II. &P<0.05. The data are presented as the mean ± standard deviation from three independent experiments. For western blot analysis, the quantification was performed by ImageJ 6.0 software; statistical significance was determined one-way ANOVA followed by the Least Significant Difference test. Ang II, angiotensin II; p, phosphorylated; NF-κB, nuclear factor-κB.