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. 2019 Oct 9;20(6):5134–5144. doi: 10.3892/mmr.2019.10733

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effects of PI3K/Akt/NF-κB signaling on the expression of nephrin and podocin mRNA and protein. Ang II reduced the expression of nephrin and podocin mRNA and protein; however, PI3K/Akt/NF-κB signaling was determined to have no effect on the expression of nephrin and podocin. (A) Reverse transcription-quantitative polymerase chain reaction of nephrin and podocin mRNA expression in 10⁻⁶ M Ang II-treated podocytes for 48 h. Compared with the control group, Ang II decreased the expression of nephrin and podocin mRNA. ^#P<0.05 vs. Control. No significant difference was observed between the control and 740Y-P and LY294002 groups. *P>0.05. Compared with the Ang II group, no significant difference was observed in the combination of Ang II + 740Y-P and Ang II + LY294002 groups. ^&P>0.05. The expression of nephrin and podocin mRNA in Ang II + 740Y-P and Ang II + LY294002 groups were decreased ^#P<0.05 vs control group (B) Western blotting of nephrin and podocin protein expression in 10⁻⁶ M Ang II-treated podocytes for 48 h. Compared with the control group, Ang II decreased the expression of nephrin and podocin mRNA. ^#P<0.05 vs. Control. No significant difference was observed between the control and 740Y-P and LY294002 groups *P>0.05. Compared with the Ang II group, no difference was observed in the combination of Ang II + 740Y-P and Ang II + LY294002 groups. ^&P>0.05. The expression of nephrin and podocin protein in Ang II + 740Y-P and Ang II + LY294002 groups were decreased ^#P<0.05 vs. control group. The data are presented as the mean ± standard deviation from three independent experiments. Statistical significance was determined one-way ANOVA. Ang II, angiotensin II; NF-κB, nuclear factor-κB.