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. 2019 Oct 17;20(6):4893–4904. doi: 10.3892/mmr.2019.10755

Figure 4.

Figure 4.

Figure 4.

Downregulation of miRNA-128-3p and upregulation of Nrf2 promote cell proliferation and inhibit apoptosis and oxidative stress. (A) Relative expression level of Nrf2 after upregulation of Nrf2 (OE-Nrf2). (B) Representative neurosphere images in NSCs subjected to OGD/R after downregulation or upregulation of miRNA-128-3p (with inhibitor and mimics, respectively), or upregulation of Nrf2 (OE-Nrf2). (C) Quantitative analysis of neurosphere diameter by Image J software. (D) Cell Counting Kit-8 (CCK-8) assay shows the cell viability of NSCs subjected to OGD/R after downregulation or upregulation of miRNA-128-3p (with inhibitor and mimics, respectively), or upregulation of Nrf2 (OE-Nrf2). (E) Flow cytometry shows the apoptosis of NSCs subjected to OGD/R after downregulation of miRNA-128-3p (Inhibitor) or upregulation of Nrf2 (OE-Nrf2). (F) Quantitative analysis of the apoptotic rate. (G) ROS levels in NSCs subjected to OGD/R after after downregulation or upregulation of miRNA-128-3p (with inhibitor and mimics, respectively), or upregulation of Nrf2 (OE-Nrf2). (H) MDA content in NSCs subjected to OGD/R after downregulation of miRNA-128-3p (Inhibitor) or upregulation of Nrf2 (OE-Nrf2). (I) GSH-Px levels in NSCs subjected to OGD/R after downregulation or upregulation of miRNA-128-3p (with inhibitor and mimics, respectively), or upregulation of Nrf2 (OE-Nrf2). (J) SOD activity in NSCs subjected to OGD/R after downregulation of miRNA-128-3p (Inhibitor) or upregulation of Nrf2 (OE-Nrf2). All assays were performed in triplicate. Data are expressed as the mean ± SD. *P<0.05, **P<0.01. Nrf2, nuclear factor (erythroid-derived 2)-related factor 2; NSCs, neural stem cells; OGD/R, oxygen-glucose deprivation and reoxygenation; MDA, malondialdehyde; GSH-Px, glutathione peroxidase; SOD, superoxide dismutase.