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. 2019 Oct 15;20(6):5041–5049. doi: 10.3892/mmr.2019.10743

Figure 4.

Figure 4.

TRAF6 is directly targeted by miR-146a. (A) Schematic diagram of the predicted target sites of miR-146a in TRAF6 3′-UTRs. The predicted target sites are underlined and mutated as indicated. (B) A549 cells were co-transfected with TRAF6 wild-type (wt) or TRAF6 mutant (mut) luciferase reporter vector (100 ng), and miR-146a mimic or NC, miR-146a inhibitor or NC inhibitor for 36 h and then harvested for luciferase assay. **P<0.01; ##P<0.01 vs. the mimic NC group or inhibitor NC group. (C and D) A549 cells were transfected with miR-146a mimic or mimic NC (C), miR-146a inhibitor or inhibitor NC (D) for 36 h and harvested for western blot analysis of TRAF6 expression. (E) A549 cells infected by H1N1 or H3N2 were treated with baicalin and transfected with blank or miR-146a mimic for 36 h and harvested for western blot analysis of TRAF6 expression. TRAF6, TNF receptor-associated factor 6.