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. 2019 Oct 16;20(6):5197–5207. doi: 10.3892/mmr.2019.10747

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of Kae on BMSC commitment to an osteogenic fate. ARS staining was performed to observe the osteogenic effects of Kae on rat BMSCs (0–100 µM). ARS-stained images (magnification, ×400) show the effect of the treatment with (A) 0, (B) 0.1, (C) 1, (D) 10 and (E) 100 µM Kae. (F) Bar graph illustrating the quantitative analysis. Values are presented as the mean ± SEM of six individual experiments. *P<0.05 vs. 0 µM. Kae, kaempferol; rBMSCs, rat bone mesenchymal stem cells; ARS, Alizarin Red S.